Digital Photodocumentation in DNA
Description Students cut DNA using restriction enzymes, run electrophoresis gels, and take digital pictures of the gels for analysis. Using a digital imaging system, students email their DNA gel pictures home for printing and further interpretation. The project allows students to apply real and practical applications in biotechnology to their biology labwork. Student Learning Outcomes Ideally, each lab group is able to document and analyze it's own data generated by the actual DNA gels run by the group. A big goal is to eliminate the need to take a Polaroid or photographic film picture of the gels; rather to capture the image digitally for further digital processing by the students. Printed hard copies are then generated to allow for interpretation of the gels. This requires precise measurements and graphing of the data off of the gel pictures. ESLR’s addressed include students demonstrating integrated technology skills, communicating effectively as well as utilizing higher level thinking skills by practical problem solving methods. By using digital images of their own stained gel, students import their images to the computer, print a hard copy, and measure physical migration of the DNA fragments off the printed image. Students will demonstrate masterey of pipetting skills including transfering of volumes of solutions as small as 1 microliter. Students will show mastery of gel loading techniques. Students will demonstrate the ability to successfully use computer software in data analysis of their DNA gels, integrating the scientific equipment with the digital software and computer.
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